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Microbiology: Lab Theory, Brief (Looseleaf)

Microbiology: Lab Theory, Brief (Looseleaf) - 2nd edition

Microbiology: Lab Theory, Brief (Looseleaf) - 2nd edition

ISBN13: 9780895829474

ISBN10: 0895829479

Microbiology: Lab Theory, Brief (Looseleaf) by Michael J. Leboffe and Burton E. Pierce - ISBN 9780895829474
Cover type: Loose-Leaf
Edition: 2ND 12
Copyright: 2012
Publisher: Morton Publishing Co.
Published:
International: No
Microbiology: Lab Theory, Brief (Looseleaf) by Michael J. Leboffe and Burton E. Pierce - ISBN 9780895829474

ISBN13: 9780895829474

ISBN10: 0895829479

Cover type: Loose-Leaf
Edition: 2ND 12


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Summary

This brief version of the best-selling laboratory manual Microbiology: Laboratory Theory and Application is intended for majors or non-majors in introductory microbiology laboratory courses. This full-color manual is appropriate for courses populated primarily by allied health students and courses with a preference for an abbreviated number of experiments.

Table of Contents

Table of Contents

1 Fundamental Skills for the Microbiology Laboratory
Exercise 1-1 A Comparison of Hand-Cleansing Agents
Exercise 1-2 Glo Germ Hand Wash Education System
Exercise 1-3 Nutrient Broth and Nutrient Agar Preparation
Exercise 1-4 Common Aseptic Transfers and Inoculation Methods
Exercise 1-5 Streak Plate Methods of Isolation
Exercise 1-6 Spread Plate Method of Isolation
2 Microbial Growth
Exercise 2-1 Ubiquity of Microorganisms
Exercise 2-2 Colony Morphology
Exercise 2-3 Growth Patterns on Slants
Exercise 2-4 Growth Patterns in Broth
Exercise 2-5 Evaluation of Media
Exercise 2-6 Fluid Thioglycollate Medium
Exercise 2-7 Anaerobic Jar
Exercise 2-8 The Effect of Temperature on Microbial Growth
Exercise 2-9 The Effect of pH on Microbial Growth
Exercise 2-10 The Effect of Osmotic Pressure on Microbial Growth
Exercise 2-11 Steam Sterilization
Exercise 2-12 The Lethal Effects of Ultraviolet Radiation on Microbial Growth
Exercise 2-13 Chemical Germicides: Disinfectants and Antiseptics
3 Microscopy and Staining
Exercise 3-1 Introduction to the Light Microscope
Exercise 3-2 Calibration of the Ocular Micrometer
Exercise 3-3 Microscopic Examination of Eukaryotic Microbes
Exercise 3-4 Simple Stains
Exercise 3-5 Negative Stains
Exercise 3-6 Gram Stain
Exercise 3-7 Acid-Fast Stain
Exercise 3-8 Capsule Stain
Exercise 3-9 Endospore Stain
Exercise 3-10 Bacterial Motility: Wet Mount and Hanging Drop Preparations
Exercise 3-11 Bacterial Motility: Flagella
Exercise 3-12 Morphological Unknown
4 Selective Media
Exercise 4-1 Phenylethyl Alcohol Agar
Exercise 4-2 Columbia CNA With 5% Sheep Blood Agar
Exercise 4-3 Mannitol Salt Agar
Exercise 4-4 MacConkey Agar
Exercise 4-5 Eosin Methylene Blue Agar
Exercise 4-6 Hektoen Enteric Agar
5 Differential Tests
Exercise 5-1 Oxidation-Fermentation Test
Exercise 5-2 Phenol Red Broth
Exercise 5-3 Methyl Red and Voges-Proskauer Tests
Exercise 5-4 Catalase Test
Exercise 5-5 Oxidase Test
Exercise 5-6 Nitrate Reduction Test
Exercise 5-7 Citrate Test
Exercise 5-8 Decarboxylation Test
Exercise 5-9 Phenylalanine Deaminase Test
Exercise 5-10 Bile Esculin Test
Exercise 5-11 Starch Hydrolysis
Exercise 5-12 Urea Hydrolysis
Exercise 5-13 Casein Hydrolysis Test
Exercise 5-14 Gelatin Hydrolysis Test
Exercise 5-15 DNA Hydrolysis Test
Exercise 5-16 Lipid Hydrolysis Test
Exercise 5-17 PYR Test
Exercise 5-18 SIM Medium
Exercise 5-19 Triple Sugar Iron Agar / Kligler Iron Agar
Exercise 5-20 Bacitracin, Novobiocin, and Optochin Susceptibility Tests
Exercise 5-21 Blood Agar
Exercise 5-22 CAMP Test
Exercise 5-23 Coagulase Tests
Exercise 5-24 Motility Test
6 Quantitative Techniques
Exercise 6-1 Standard Plate Count (Viable Count)
Exercise 6-2 Urine Culture
Exercise 6-3 Plaque Assay of Phage Titer
Exercise 6-4 Differential Blood Cell Count
Exercise 6-5 Environmental Sampling: The RODAC Plate
7 Medical Microbiology Introduction
Exercise 7-1 Snyder Test
Exercise 7-2 Antimicrobial Susceptibility Test (Kirby-Bauer Method)
Exercise 7-3 MMWR Assignment
Exercise 7-4 Epidemic Simulation
Exercise 7-5 Membrane Filter Technique
Exercise 7-6 Multiple Tube Fermentation Method for Total Coliform Determination
Exercise 7-7 Methylene Blue Reductase Test
Exercise 7-8 Making Yogurt
8 Microbial Genetics and Serology
Exercise 8-1 Extraction of DNA from Bacterial Cells
Exercise 8-2 Ultraviolet Radiation Damage and Repair
Exercise 8-3 Bacterial Transformation: The pGLO System
Exercise 8-4 Slide Agglutination
Exercise 8-5 Blood Typing
Exercise 8-6 ELISA For Detecting Antibodies in a Patient's Sample (The Antibody Capture Method)
9 Identification of Unknowns
Exercise 9-1 Identification of Enterobacteriaceae
Exercise 9-2 Identification of Gram-positive Cocci
Exercise 9-3 Identification of Gram-positive Rods
Exercise 9-4 API 20 E Identification System for Enterobacteriaceae and Other Gram-negative Rods
Exercise 9-5 Enterotube II
Exercise 9-6 Identification of Streptococcus and Streptococcus-like Organisms by Latex Agglutination: The Streptex Rapid Test

Appendix A: Biochemical Pathways
Appendix B: Miscellaneous Transfer Methods
Appendix C: Transfer from a Broth Culture Using a Glass Pipette
Appendix D: Transfers from a Broth Culture Using a Digital Pipette
Appendix E: Media, Reagent, and Stain Recipes
Glossary
Index

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